Glycosyl compounds
![Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 250 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B71800-0.25.JPG-250.jpg)
Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 250 Milligrams
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture
FUJIFILM Biosciences CultureSure 5mmol/L SB431542 D
This is a ready-to-use 5 mmol/L solution of SB431542 in dimethyl sulfoxide DMSO specifically formulated as animal-SB431542 inhibits ALK4 5 7 Reports on the simulation of formation of mono-layers differentiation and proliferation of embryonic stem cell-derived endothelial cells Otherwise this reagent when used together with thiazovivin and PD0325901 increases the reprogramming efficiency by 200 fold as well as accelerates reprogramming Ogawa K et al J Cell Sci 120 55 2007 Lin T et al Nat Methods 6 805 2009
Cayman Chemical Trehalose 6 6-DIbehenate 25mg
A synthetic glycolipid and derivative of TDM; binds to a fusion protein of mouse Mincle and human IgG1 Fc in a cell-free assay at 10 UG/ml; induces NO and G-CSF production in mouse bone marrow macrophages in a Mincle-dependent manner at 5 UG/ml; liposomes containing TDB and DDA as adjuvants and Ac2SGL and PIM2 decrease spleen bacterial load and the number of lung and spleen lesions in a guinea pig model of M. tuberculosis infection